Coding
HADase 1
Part:BBa_K2742000:Design
Designed by: Frank Loeffler, Brandon Kristy, and Karl Leitner Group: iGEM18_UT-Knoxville (2018-10-09)
HADase 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized, and expresses well within E. coli
Source
The sequence originates from the genome of Kyrpidia sp. EA-1; an aerobic bacteria obtained from hydrothermal sediment samples. The HADase gene of interest was synthesized from IDT.